Journal: Cell Death & Disease

Article Title: Ficolin B secreted by alveolar macrophage exosomes exacerbates bleomycin-induced lung injury via ferroptosis through the cGAS-STING signaling pathway

doi: 10.1038/s41419-023-06104-4

Figure Lengend Snippet: A The concentrations of L-ficolin and M-ficolin in plasma were measured by biochemical kits. *ILD vs. Healthy control, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. B The Survival curves of mice after BLM induction. C The histopathological changes in the lung were observed by HE staining. D Pulmonary interstitial fibrosis was detected by Masson staining. E , F The concentrations of total protein in BALF and hydroxyproline in lung were determined by biochemical kits. G The expression level of α-SMA in lung was detected by Western blot. H , I The percentage of Fcn B positive cells in lung macrophages and neutrophils was detected by flow cytometry. The experiment was repeated three times ( n = 10 mice/group). The data were presented as the mean ± SD. *WT+BLM vs. WT, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; # Fcnb -/- +BLM vs. WT+BLM, # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001.

Article Snippet: The concentrations of total protein in bronchoalveolar lavage fluid (BALF) (AWB0104, Abiowell, China), hydroxyproline (CSB-E08839m, CUSABIO, China), malondialdehyde (MDA) (A003-1-2, NJJCBIO, China), glutathione (GSH) (A006-2-1, NJJCBIO, China), Fe 2+ (TC1015, Leagene, China), L-ficolin (Ab213778, Abcam, UK), and M-ficolin (Ab213777, Abcam, UK) were measured according to the instructions of kits.

Techniques: Clinical Proteomics, Control, Staining, Expressing, Western Blot, Flow Cytometry

Journal: Cell Death & Disease

Article Title: Ficolin B secreted by alveolar macrophage exosomes exacerbates bleomycin-induced lung injury via ferroptosis through the cGAS-STING signaling pathway

doi: 10.1038/s41419-023-06104-4

Figure Lengend Snippet: A The histopathological changes in the lung were observed by HE staining. B Pulmonary interstitial fibrosis was detected by Masson staining. C , D The concentrations of total protein in BALF and hydroxyproline in the lung were determined by biochemical kits. E The expression of α-SMA in lung was detected by Western blot. The experiment was repeated three times ( n = 10 mice/group). The data were presented as the mean ± SD. * WT+BLM vs. WT, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; $ WT+BLM+Exo oe-NC vs. WT+BLM, $ P < 0.05, $$ P < 0.01, $$$ P < 0.001, $$$$ P < 0.0001; & WT+BLM+Exo oe-Fcnb vs. WT+BLM+Exo oe-NC , & P < 0.05, && P < 0.01, &&& P < 0.001, &&&& P < 0.0001; # Fcnb -/- +BLM vs. WT+BLM, # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001; @ Fcnb -/- +BLM+Exo oe-NC vs. Fcnb -/- +BLM, @ P < 0.05, @@ P < 0.01, @@ @ P < 0.001, @@@@ P < 0.0001; Δ Fcnb -/- +BLM+Exo oe-Fcnb vs. Fcnb -/- +BLM+Exo oe-NC , Δ P < 0.05, ΔΔ P < 0.01, ΔΔΔ P < 0.001, ΔΔΔΔ P < 0.0001.

Article Snippet: The concentrations of total protein in bronchoalveolar lavage fluid (BALF) (AWB0104, Abiowell, China), hydroxyproline (CSB-E08839m, CUSABIO, China), malondialdehyde (MDA) (A003-1-2, NJJCBIO, China), glutathione (GSH) (A006-2-1, NJJCBIO, China), Fe 2+ (TC1015, Leagene, China), L-ficolin (Ab213778, Abcam, UK), and M-ficolin (Ab213777, Abcam, UK) were measured according to the instructions of kits.

Techniques: Staining, Expressing, Western Blot

Journal: European journal of pharmacology

Article Title: Chromofungin, a chromogranin A-derived peptide, protects against sepsis-induced acute lung injury by inhibiting LBP/TLR4-dependent inflammatory signaling.

doi: 10.1016/j.ejphar.2023.176043

Figure Lengend Snippet: Fig. 1. Effects of CHR pretreatment on pulmonary inflammation in septic mice. Mice were subjected to CHR pretreatment and CLP administration as described in the “Materials and Methods” section. (A) Structure diagram of CHR. (B) Survival rates of mice in the Sham (n = 18), CLP (n = 18), CHR + Sham (n = 17), and CHR + CLP groups (n = 16). Eighty mice were randomly divided into four groups with twenty mice in each group, excluding mice that died during anesthesia and resuscitation. (C–E) Protein levels of IL-1β, IL-4, IL-10 in BALF (C), mRNA levels of TNF-α, IL-1β, IL-4 and IL-10 in lung tissues (D), images of H&E-stained lung tissues (200x) and semiquantitative histological scores of lung injury (E) from mice with or without treatment 30 min after intraperitoneal CHR injection, followed by sham or CLP administration. One-way ANOVA followed by multiple comparison tests were used to analyze the data, and the data represent the mean ± SEM (n = 3). The sig nificance level was adjusted at 0.05. Scale bar = 50 μM *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: Cytokine (IL-1β, interleukin-4 [IL-4] and interleukin-10 [IL-10]) levels in mouse bronchoalveolar lavage fluid (BALF) or cell culture supernatants were measured by a mouse ELISA detection kit (Proteintech).

Techniques: Staining, Injection, Comparison

Journal: Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology

Article Title: Inhalation of nebulized Mycobacterium vaccae can protect against allergic bronchial asthma in mice by regulating the TGF-β/Smad signal transduction pathway

doi: 10.1186/s13223-020-00456-8

Figure Lengend Snippet: Cells count in BALF. # P < 0.01, compared with Normal group; ## P < 0.01. compared with Asthma control group

Article Snippet: The lungs were lavaged using 500 μL iced PBS thrice, and the bronchoalveolar lavage fluid (BALF) was collected in Eppendorf tubes on ice.

Techniques: Control